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. 2017 Nov 14;7:15535. doi: 10.1038/s41598-017-15592-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Mutations in the ND1 gene that alter evolutionarily conserved amino acid residues. (A) Images of haematoxylin and eosin (HE) staining of tumour lesion and adjacent normal tissues of colon cancer (ColoCa-7) are shown. Electropherograms depict the T3394C (Y30H) SNV. The SNV is present in the tumour lesion but not in the adjacent mucosal tissue. The evolutionary conservation of the tyrosine residue is shown. (B) Electropherograms showing the T3398C (M31T) SNV. The evolutionary conservation of the histidine residue is shown. (C) Electropherograms showing the G3709A (A135T) SNV. The evolutionary conservation of the alanine residue is shown.