Figure 4.

Role of LasR quorum sensing in the deleterious effect of P. aeruginosa on F508del-CFTR maturation and functional rescue by VRT-325. CFBE-ΔF508 cells treated or not with the VRT-325 corrector (5 μM) were exposed to LB or exoproducts from PAO1-V or PAO1-V mutated in the lasR gene (PAO1-VΔlasR) for 24 h. (A) Representative immunoblots (left) and densitometric analysis (right, reported as % of LB control) of mature (band C) CFTR protein expression levels (n = 10). (B) Representative traces of short-circuit current (I_sc) measurements in Ussing chamber and quantification of mean CFTR_Inh172 (20 μM)-sensitive currents (ΔI_{CFTRInh−172}) (C) through CFBE-ΔF508 cells, in each condition (n = 13). NS, non-significant, ^*p < 0.05, ^**p < 0.01, ^***p < 0.001.