Figure 5.

Quorum sensing inhibition abrogates the negative effect of P. aeruginosa on F508del-CFTR maturation and functional rescue with VRT-325. CFBE-ΔF508 cells were treated or not, for 24 h, with VRT-325 and LB or exoproducts from PAO1 bacteria cultured, or not, with 0.125 mg/ml of HDMF. (A) Representative immunoblots (left) and densitometric analysis (right, reported as % of LB control) of mature (band C) CFTR protein expression levels (n = 7). (B) Representative traces of short-circuit current (I_sc) measurements in Ussing chamber and quantification of the mean CFTR_Inh172 (20 μM)-sensitive currents (ΔI_{CFTRInh−172}) (C) through CFBE-ΔF508 cells, in each condition (n = 9). NS, non-significant, ^**p < 0.01, ^***p < 0.001.