Figure 7.

Improved CFTR rescue by Vx-809 in primary airway epithelial cells from CF patients with F508del mutations after P. aeruginosa quorum sensing inhibition. Short-circuit currents measurements were performed on human primary airway cells from 4 different CF patients homozygous for the F508del mutation cultured at the air-liquid interface for 35 days and then treated, or not, for 24 h with 5 μM Vx-809 and LB or exoproducts from PAO1 cultured with (PAO1+HDMF+Vx-809) and without (PAO1+Vx-809) 0.125 mg/ml HDMF. LB was used as control (LB and LB+Vx-809 conditions). CFTR_Inh172 (20 μM)-sensitive currents (ΔI_{CFTRInh−172}), in each condition, are presented individually, for each patient.