Table 1.
The primers used of PCR.
| Primers | Sequence (5′-3′) | Usage |
|---|---|---|
| Pttrpc01 | ATGTCCTCGTTCCTGTCTGCTAATA | First PCR reaction for T-DNA left border |
| LB-1 | AGGGTTCCTATAGGGTTTCGCTCAG | |
| Pttrpc01 | ATGTCCTCGTTCCTGTCTGCTAATA | Second PCR reaction for T-DNA left border |
| LB-3 | GAATTAATTCGGCGTTAATTCAGT | |
| N1812SalI L1 | AGTCGCCAACAACAGGAT | First PCR reaction for gene left border |
| N1812 LB1 | CCATCATTTGGGCTGTAAG | |
| N1812SalI L2 | AGTCGCCAACAACAGGAT | Second PCR reaction for gene left border |
| N1812 LB2 | ACTGAGGGTTGTCGTGTTC | |
| N1812HindIIIR1 | GCTTGTAAACCTGGACCCT | First PCR reaction for gene right border |
| N1812RB1 | GAAGTGGAGTAAACGACCTG | |
| N1812HindIIIR2 | GCTTGTAAACCTGGACCCT | Second PCR reaction for gene right border |
| N1812RB2 | CTTTAGGCAAGCCCACAT | |
| HPH-SP | TTCTGCGGGCGATTTGTG | The probe for T-DNA copy number |
| HPH-AP | AGCGTCTCCGACCTGATG | |
| N1812LBFP1(EX) | TAAGTAGAACACGACAACCCTC | The probe for CmSIT1 gene copy number |
| N1812LBFP2(EX) | TGCCTAAAGTAACGCAGATT | |
| SIT1SP(EX) | CCACTTCCAACCCGACAC | CmSIT1 primers for RT- PCR |
| SIT1AP(EX) | CTTACGCCTCCGACAAAT | |
| PEX14-3′RACE2 | CGCCCTCGGTGAAATGGA | CmPEX14 primers for RT- PCR |
| P-AP(EX) | AAGAGCCTTGGGAATGAGAT | |
| Actin-FP | ACCGTGAGAAGATGACCC | Actin primers for RT- PCR |
| Actin-RP | AAGGACAGAAGGCTGGAAG | |
| qRT-PCR-FP1 | TCCTGTTGTTGGCGACTC | CmSIT1 primers for qRT-PCR |
| qRT-PCR-RP1 | CGGCAGCGACAAGAGTAG | |
| CmACT289 | GTCCGTGACATCAAGGAGAAGC | Actin primers for qRT-PCR |
| CmACT419 | TT GCCAATGGTGATGACCTGAC | |
| SIT1-SP(OVER-T) | GGATCCCAATGACGGACACCGAAA | CmSIT1 cDNA |
| SIT1-AP(OVER-T) | ATCGATGCTTGTAAACCTGGACCCT |