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. 2017 Nov 10;8:2216. doi: 10.3389/fmicb.2017.02216

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Copyright © 2017 He, Wang, Wang, Zhang and Tian.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Exogenous application of cAMP fails to restore appressoria formation in the CgMK1 mutants. (A) Conidial germination and appressoria phenotype induced by addition of 10 mM cAMP on the hydrophobic surface of GelBond membrane. Scale bar: 15 μm. (B) The germination rate of the strains on the hydrophilic surface of the GelBond membrane in the presence or absence of 10 mM cAMP at 25°C for 4 h. Error bars represent the standard deviations based on three independent replicates with three technical replicates each.