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. 2017 Nov 10;8:2216. doi: 10.3389/fmicb.2017.02216

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Copyright © 2017 He, Wang, Wang, Zhang and Tian.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Assays for penetration and colonization defects in the CgMK1 mutants. (A) Onion epidermal cell penetration assay of the ΔCgMK1 mutants. The assay was performed by inoculating conidial suspensions from the indicated strains. Examination under a light microscope was performed after aniline-blue staining. A: appressorium; C: conidium; IH: invasive hyphae. Scale bar: 10 μm. (B) Cellophane membranes penetration assay was performed by the conidial suspensions from strains. Colonies of the indicated strains grown on PDA plates covered with a cellophane membrane (before). Then the medium was removed the cellophane membrane and placed for additional days (after). Photos in the first row were taken at 3 dpi, the second row were taken at 5 dpi that showed growth of strains after penetration from cellophane membrane.