Table 1.
PCR primers used in this study.
| Primer name | Sequence | Use in this study |
|---|---|---|
| CgMK1-5Ffor | CTTACCTGGCCTCGTCGTTT | 5F flanking sequence |
| CgMK1-5Frev | GATACTACTTCGGGCGGAGC | |
| CgMK1-3Ffor | TCCCATTCCGGAGGAGTTCT | 3F flanking sequence |
| CgMK1-3Frev | CGCTTCAAAGCCTTCCCCTA | |
| External-CgMK1for | CACCGACCAAGGCAATTGTG | External sequence used for validation of mutant |
| External-CgMK1rev | GCATTGGTGTGCCGTTCTTT | |
| CgMK1-Compfor | CGTCCTTTTGCGCACCTTAC | Complementary sequence |
| CgMK1-Comprev | TAACCTCGAAGACCCTTGCG | |
| Promoter + CgMK1rev | CCGCATAATCTCCTGGTAAA | Complementary sequence without stop codon |
| Promoter + CgMK1 + eGFPrev | ACGCTCTTTTCTCTTAGGTTTA | Complementary and sublocation sequence cassette |
| RT-CgMK1for | TGCTTAGTGGAAAGCCCCTG | qRT-PCR of CgMK1 |
| RT-CgMK1rev | AGCGAATGTACTCTCTGGCG | |
| Cg18S-up | GTGAGGCCCTCAAAGGTAGTGG | qRT-PCR of Cg18S |
| Cg18S-down | GGATCCCAGTGCGAGACGT | |
| ProbeHPHfor | AAAGAACGGCACACCAATGC | Probe HPH sequence used for hybridization |
| ProbeHPHrev | ATGAGAGAAACGACCAGGCG | |
| ProbeCgMK1for | GCGAAGAATCTCGTGCTTTC | Probe CgMK1 sequence used for hybridization |
| ProbeCgMK1rev | GATGTTGGCGACCTCGTATT | |
| YG-F | GATGTAGGAGGGCGTGGATATGTCCT | The 2/3rd portion of the hygromycin cassette |
| HY-R | GTATTGACCGATTCCTTGCGGTCCGAA | |
| Hygromycinfor | CGCCAGGGTTTTCCCAGTCACGAC | hygromycin cassette |
| Hygromycinrev | AGCGGATAACAATTTCACACAGGA |