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. 2017 Nov 14;7:15578. doi: 10.1038/s41598-017-15790-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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System calibration and optical property correction. (a) and (b) show absorption maps of one calibration phantom at 490 nm and 590 nm respectively. The dashed square indicates the ROI. (c) and (d) show scattering maps of the same calibration phantom at 490 nm and 590 nm respectively. (e) and (f) show the quantification of absorption and scattering all four calibration phantoms respectively. (g) A schematic of the acquired phantom optical properties. (h) Uncorrected (raw) Dox fluorescence vs. Dox concentration, showing the large variation with respect to background optical property differences. (i) Attenuation-corrected Dox fluorescence vs. Dox concentration using all phantoms at different absorption and scattering parameters, indicating a strong linear relationship with respect to Dox concentration. Error bars represent standard deviation between phantoms.