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. 2017 Nov 14;7:15553. doi: 10.1038/s41598-017-15854-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Workflow for large-scale detection of K_his modification sites in P. patens. (A) Western blotting analysis of total protein extracts to investigate the existence of K_hib-modified proteins in P. patens. (B) The experimental strategy to identify K_hib modification sites in P. patens. Total protein extracted from P. patens was digested by trypsin. Peptides containing K_hib modification sites were enriched by immunoprecipitation with a K_hib-specific antibody and subsequently analyzed by LC-MS/MS.