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. 2017 Nov 14;7:15521. doi: 10.1038/s41598-017-15748-0

Figure 2.

Figure 2

Mutations disrupt Z-domain interaction. IgG1 variants were eluted from a column comprised of Z-domain using a 30 mL gradient from pH 7.2 to pH 3.0 (dotted line). Variants are indicated on the charts. Absorbance at 280 nm was monitored during elution (solid lines).