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. 2017 Nov 14;7:15581. doi: 10.1038/s41598-017-15833-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Inhibition of classic estrogen receptors and ALK2/3 together reduces somatic cell differentiation and inhibits PF formation. Primordial follicle index in E15 ovary after culture with either vehicle or BMP2 plus E2 from C0-C8 in the presence or absence of LDN 193,189, an ALK1/2 inhibitor and ICI 182,780, an ESR inhibitor from C6-C8. Each bar represents the PF index ± SEM, n = 4. Data were analyzed by one-way ANOVA with Tukey’s post-hoc test. P < 0.05, bars with different letter, P > 0.05, bars with same letter.