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. 2017 Nov 14;7:15575. doi: 10.1038/s41598-017-15586-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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FAM19A5 regulates RANKL-induced gene expression during osteoclastogenesis. (A,C,D) Mouse BMDMs were stimulated with FAM19A5 (2 μM) in the presence of M-CSF (30 ng/ml) and RANKL (100 ng/ml) for 3 days. Cells were harvested for RNA preparation. qPCR was performed using specific primers for RANK, TRAF6, OSCAR, TRAP, Blimp1, c-FOS, NFATc1, MafB, IRF-8, DC-STAMP, OC-STAMP, Atp6v0d2, and GAPDH. (B) Cells were harvested and Western blot analysis was conducted using anti-RANK, anti-TRAF6, anti-c-fos, anti-NFATc1, and β-actin antibodies. Data are presented as means ± SE (n = 3). Data are representative of three independent experiments (A,C,D). *p < 0.05; **p < 0.01; ***p < 0.001. Results shown are representative of three independent experiments (B).