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. 2017 Nov 14;7:15575. doi: 10.1038/s41598-017-15586-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Inhibitory effects of FAM19A5 on RANKL-induced osteoclast formation are mediated by FPR2. (A–C) Mouse BMDMs were pre-incubated with PTX (100 ng/ml), WRW4 (60 μM) or cyclosporin H (10 μM) prior to FAM19A5 (2 μM) treatment in the presence of M-CSF (30 ng/ml) and RANKL (100 ng/ml) for 4 days. All cells were stained with TRAP staining solution. Osteoclasts were considered for TRAP+ 3 < MNCs. Data are presented as means ± SE (n = 3). Data are representative of at least two independent experiments. ns: not significant, **p < 0.01.