FIGURE 5.

Analysis of LasA activities and pyocyanin production in P. aeruginosa acpP1 mutant strains. (A) LasA protease activities in culture supernatants of P. aeruginosa strains. Empty circle indicates boiled Staphylococcus aureus cells; filled square indicates boiled S. aureus cells containing culture supernatants of P. aeruginosa mutant strain PA-A1; filled circle indicates boiled S. aureus cells containing culture supernatants of P. aeruginosa PAO1; empty triangle indicates boiled S. aureus cells containing culture supernatants of complementary strain PK-Pa. (B) Pyocyanin in culture supernatants of P. aeruginosa strains. Data are the mean ± standard deviation of triplicate measurements. Pair-wise comparisons were made between wild type strain PAO1 and mutant strain PA-A1 or complementary strain PK-Pa by Student’s t-test. ^∗∗∗Highly significant difference, P < 0.01.