Table 3.
SAXS data collection and scattering-derived structural parameters (at the highest concentration) for HvASR1 and TtASR1.
| HvASR1 | TtASR1 | |
|---|---|---|
| Data-collection parameters | ||
| Detector | Pilatus (1 M) | Pilatus (1 M) |
| Beam geometry | Bending magnet (BM29) | Bending magnet (BM29) |
| Wavelength (Å) | 0.992 | 0.992 |
| q Range (Å−1) | 0.028–4.525 | 0.028–4.525 |
| Exposure time (s) | 1 | 1 |
| Concentration range (g/L) | 1–1.5 | 1–3 |
| Temperature (°C) | 20 | 20 |
| Structural parameters | ||
| I(0) (cm−1) (from Guinier) | 12.7 ± 0.8 | 12.5 ± 0.5 |
| Rg (nm) (from P(r)) | 3.57 ± 0.02 | 3.58 ± 0.05 |
| Rg (nm) (from Guinier) | 3.46 ± 0.06 | 3.55 ± 0.03 |
| Dmax (nm) | 11.9 | 12.3 |
| Molecular-mass determination (kDa) | ||
| Molecular mass (MM) (from I(0)) | 12.8 | 13 |
| Calculated MM from sequence | 15.9 | 15.7 |
| Software employed | ||
| Primary data reduction | PRIMUS | PRIMUS |
| Data processing | GNOM | GNOM |
| Validation of structural models | CRYSOL/GAJOE | CRYSOL/GAJOE |