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. 2017 Nov 14;7:15495. doi: 10.1038/s41598-017-15707-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Phylogenetic trees of 185 GPCR sequences using both character-based (a) and distance-based methods (b). The data points in (c) show the location of these sequences on a two-dimensional projection by MDS. The branching tree diagram in (a) was constructed using the maximum likelihood method and displayed with the polar tree layout. The minimum spanning tree diagram in (b) was displayed by viewing each level 1 MSC cluster as a node.