Figure 5.

Grx1 mediates the effect of s-flow against apoptosis through inhibition of H₂O₂-induced JNK phosphorylation. (A) Equal amounts of total proteins were used to measure Grx1 activity. Grx1 activity was measured by Grx1 activity assay. One unit of Grx1 activity was defined as 1 µmol of NADPH oxidized per min under the standard assay conditions (data were expressed as mean ± SEM, n = 3). (B,C) Grx1 siRNA was transfected into HUVEC, which were subjected to s-flow before exposed to H₂O₂. After 1 h, cell lysates were subjected to SDS-PAGE and immunoblotting with the indicated antibodies. *p < 0.05.