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. 2017 Nov 14;7:15588. doi: 10.1038/s41598-017-15783-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cytopathic effect induced by WT MCMV or mutant virus. (a) Monolayers of C127I epithelial cells infected with WT MCMV or the ΔM24-m25.2 mutant were visualized 72 h p.i. by fluorescence and light microscopy. Foci of infected cells were detected utilizing virus-driven GFP expression. (b) The scheme illustrates the genome structure of the mutants. The HindIII cleavage map of the MCMV Smith strain genome is depicted at the top and the genomic region encompassing ORFs M24 to M26 is shown enlarged below. , kanamycin resistance gene; ---, deleted sequences; → , orientation of ORFs; ▪, FRT sites. (c) Cells infected with the indicated viruses were examined by fluorescence microscopy 48 h p.i. Scale bars, 100 µm.

Inline graphic — Cytopathic effect induced by WT MCMV or mutant virus. (a) Monolayers of C127I epithelial cells infected with WT MCMV or the ΔM24-m25.2 mutant were visualized 72 h p.i. by fluorescence and light microscopy. Foci of infected cells were detected utilizing virus-driven GFP expression. (b) The scheme illustrates the genome structure of the mutants. The HindIII cleavage map of the MCMV Smith strain genome is depicted at the top and the genomic region encompassing ORFs M24 to M26 is shown enlarged below. , kanamycin resistance gene; ---, deleted sequences; → , orientation of ORFs; ▪, FRT sites. (c) Cells infected with the indicated viruses were examined by fluorescence microscopy 48 h p.i. Scale bars, 100 µm.