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. 2017 Nov 14;7:15588. doi: 10.1038/s41598-017-15783-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Subcellular localization of M25 proteins during the MCMV infection cycle. (a,b) MEF infected with the vM25HA virus for the indicated time periods were analyzed by confocal microscopy after labeling with antibodies specific for the HA epitope, lamin B and the Golgi protein GM130. Size bar, 10 µm. (c) NIH 3T3 cells were infected with the vM25HA virus. At the indicated time points either total cell lysates (T) or cytoplasmic (C) and nuclear (N) fractions were prepared and analyzed by immunoblotting with the M25-specific antibody. Fractionation was verified for lamin B and β-tubulin.