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. 2017 Nov 14;7:15588. doi: 10.1038/s41598-017-15783-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Viral gene expression and genome replication are not affected by the lack of M25 proteins. (a) NIH 3T3 cells were either mock-infected or infected with the indicated viruses (MOI 3.) At the indicated time points lysates were prepared and analyzed by immunoblotting using antibodies specific for the viral immediate-early protein IE1, early protein E1, or glycoprotein B. Vimentin served as loading control. (b) MCMV genome copy numbers present in NIH 3T3 cells (infected as described in (a)) were measured by qPCR at the indicated time points. Graph shows means ± SD of 2 to 3 independent experiments.