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. 2017 Nov 14;7:15498. doi: 10.1038/s41598-017-15628-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Influence of different point mutations on the concentration dependence of GABA. Wild type or mutant β₂γ₂ GABA_A receptors were expressed in Xenopus oocytes and exposed to subsequently higher concentrations of GABA and the elicited current amplitude was determined. Individual curves for each subunit combination were first normalized to the fitted maximal current amplitude and subsequently averaged. Averaged concentration-response curve are shown. Data are expressed as mean ± S.E.M., n = 3–7 from two batches of oocytes.