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. 2017 Nov 14;7:15574. doi: 10.1038/s41598-017-15773-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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UV irradiation of mouse skin decreased adult hippocampal neurogenesis. Representative immunohistochemical images of (a) DCX-positive and (b) Ki-67-positive cells in the DG. DCX- and Ki-67-positive cells were counted in 6 hippocampal sections from each mouse and in 8 mice from each group, and mean values were calculated. (c) The protein expression levels of DCX, Bcl-2, and cleaved caspase-3 were assessed in hippocampal lysates using Western blot. Relative band density was analyzed using Image J software. Tubulin was used as the endogenous control. The blots are cropped raw images are provided in Supplementary Figure S2. Scale bars: 100 µm. Graphs show means ± SEM (n = 8 mice/group). *P < 0.05 and ***P < 0.001 indicate significant differences when compared to the control group. Bcl-2, B-cell lymphoma 2; C.caspase-3, cleaved caspase-3; DCX, doublecortin; DG, dentate gyrus.