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. 2017 Nov 14;7:15552. doi: 10.1038/s41598-017-15791-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of CerS6 ablation on proliferation. (a) Splenocytes from wild type and CerS6-deficient littermates were activated and clonal expansion assessed through cell counting. (b) SW480 cells −/+ shRNA against CerS6 were grown in the absence or presence of 10 μg/ml doxycycline for 4 days. Knockdown of CerS6 was verified by Western blot and proliferation assessed using the CellTiter-Blue assay. Values were normalized to cells grown in the absence of doxycycline. n = 2.