Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Nov 14;7:15528. doi: 10.1038/s41598-017-14764-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

YAP and LRP1’s function in promoting transformative phenotypes in melanoma A375 cells. (a,b) Cell proliferation of melanoma A375 cells under infection of YAP-sh plasmid or LRP1-sh plasmid as indicated were evaluated using MTT assay. The initial cell number is 5000 for MTT assay, and the data from the “GFP-sh” group were arbitrarily set to 100%. (c,d) Caspase 3/7 activities of melanoma cells under infection of YAP-sh plasmid or LRP1-sh plasmid as indicated were measured by a Caspase-Glo 3/7 assay kit from Promega. (e) YAP and LRP1 reduced Caspase 3 expression, as measured by immunofluorescence assay, in A375 cells infected of YAP-sh plasmid or LRP1-sh plasmid for 24 h. Scale bar.μm. (f,g) Cell proliferation of melanoma A375 cells under infection of YAP-sh plasmid or LRP1-sh plasmid as indicated were evaluated using transwell assay. The initial cell number is 5000 for transwell assay, and the data from the “GFP-sh” group were arbitrarily set to 100%. (h) Cell proliferation of melanoma A375 cells under transfection of YAP-FLAG plasmid or LRP1-FLAG plasmid as indicated were evaluated using MTT assay respectively. (i) Caspase 3/7 activities of melanoma cells under transfection of YAP-FLAG plasmid or LRP1-FLAG plasmid as indicated were measured by a Caspase-Glo 3/7 assay kit from Promega. (j,k) Cell proliferation of melanoma A375 cells under transfection of YAP-FLAG plasmid or LRP1-FLAG plasmid as indicated were evaluated using MTT assay. Data were shown as mean ± SD from three independent experiments (including WB). *P < 0.05; **P < 0.01; ***P < 0.001 versus control measured by the student t test.