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. 2017 Nov 14;7:15528. doi: 10.1038/s41598-017-14764-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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YAP and LRP1’s function in promoting transformative phenotypes in melanoma MUM-2B cells. (a,b) Cell proliferation of melanoma MUM-2B cells under infection of YAP-sh plasmid or LRP1-sh plasmid as indicated were evaluated using MTT assay respectively. The initial cell number is 5000 for MTT assay, and the data from the “GFP-sh” group were arbitrarily set to 100%. (c,d) Caspase 3/7 activities of melanoma cells under infection of YAP-sh plasmid or LRP1-sh plasmid as indicated were measured by a Caspase-Glo 3/7 assay kit from Promega. (e) YAP and LRP1 reduced Caspase 3 expression, as measured by immunofluorescence assay, in MUM-2B cells infected of YAP-sh plasmid or LRP1-sh plasmid for 24 h. Scale bar.μm. (f,g) Cell proliferation of melanoma MUM-2B cells under infection of YAP-sh plasmid or LRP1-sh plasmid as indicated were evaluated using transwell assay. The initial cell number is 5000 for transwell assay, and the data from the “GFP-sh” group were arbitrarily set to 100%. (h) Cell proliferation of melanoma MUM-2B cells under transfection of YAP-FLAG plasmid or LRP1-FLAG plasmid as indicated were evaluated using MTT assay. (i) Caspase 3/7 activities of melanoma cells under transfection of YAP-FLAG plasmid or LRP1-FLAG plasmid as indicated were measured by a Caspase-Glo 3/7 assay kit from Promega. (j,k) Cell proliferation of melanoma MUM-2B cells under transfection of YAP-FLAG plasmid or LRP1-FLAG plasmid as indicated were evaluated using transwell assay. Data were shown as mean ± SD from three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001 versus control measured by the student t test.