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. 2017 Nov 14;7:15510. doi: 10.1038/s41598-017-15332-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Food anticipatory activity from individual wild-type and Period mutant (Brdm strain) mice. (a) Food anticipatory activity (FAA) during 9 days of restricted feeding (RF) of wild-type (n = 12) and mPer2 ^Brdm1−/− (n = 17) mice was determined by totaling the number of wheel revolutions per minute from 4 hours before feeding time to the end of feeding time (total of 8 hours). FAA during fasting was defined as the total number of wheel revolutions per minute from 4 hours before feeding time to the end of previous feeding time (total of 8 hours). Wheel-running FAA for each mouse was determined separately for the first (Day 1; b) or second (Day 2; c) day of fasting. Black circles are individual mice fed from ZT6-10. Blue circles are individual mice fed from ZT5-9. The mean of each group is a horizontal line.