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. 2017 Nov 14;7:15551. doi: 10.1038/s41598-017-15861-0

Figure 2.

Figure 2

Low-salt versus high-salt perception and the role of the NO/sGC/cGMP cascade. Low-salt solution (a–d) or high-salt solution (e–h) were offered to insects. (a,e) Feeding index of insects previously treated with control solution (CS), SNAC (NO donor), ODQ (sGC inhibitor), 8-Br-cGMP (cGMP analogue) or PTIO (NO scavenger). (b,c,f,g) EMG examples of insects feeding on low-Na when previously treated with CS (b) or SNAC (c) and of insects feeding on high-salt but previously treated with CS (f) or PTIO (g). EMG recordings lasted 10 minutes, although only 20 seconds are shown. (d,h) Pumping frequency of the cibarial musculature (in Hz, pumps per second) of treated insects with CS, ODQ, SNAC, 8-Br-cGMP or PTIO. Different letters denote significant differences (p < 0.05). Numbers of insects used are shown in brackets. B = insertion of mouthparts in the feeder, W = withdrawal of mouthparts.