Table 1.
Target | Species | Vendor | Cat number | Dilution1 | Concentration (µg/ml) | HIER buffer | Label (enzyme) | Incubation | Panel |
---|---|---|---|---|---|---|---|---|---|
CK18 | ms | SantaCruz | 6259 | 2000 | 0.1 | Tris-EDTA | TSA488 | o/n | EPI |
CK8 | ms | Invitrogen | 18–0185z | 2000 | 0.11 | Tris-EDTA | TSA555 | o/n | EPI |
p63 | rbt | Abcam | 124762 | 1000 | 0.87 | Tris-EDTA | AlexaFluor647 | 90 min | EPI |
CK5 | rbt | Abcam | 52635 | 400 (2000) | 0.88 | Tris-EDTA | AlexaFluor647 | 90 min | EPI |
E-cadherin | ms | BD biosciences | 610182 | 1000 (10000) | 0.25 | Tris-EDTA | AlexaFluor750 | 90 min | EPI |
PanCK | ms | Abcam | 7753 | 200 (2000) | 5 | Tris-EDTA | AlexaFluor750 | 90 min | EPI |
PanCK | ms | Invitrogen | 18–0132 | 200 (2000) | 0.18 | Tris-EDTA | AlexaFluor750 | 90 min | EPI |
AR | rbt | SantaCruz | 13062 | 2000 | 0.1 | Tris-EDTA | VinaGreen (HRP) | 90 min | EPI |
AMACR | ms | Abcam | 63340 | 2000 | N/A, LOT GR154665-12 | Tris-EDTA | Liquid Permanent Red (AP) | 90 min | EPI |
FoxP3 | ms | Abcam | 20034 | 500 | 2 | Tris-EDTA | TSA488 | o/n | IC |
CD4 | ms | Abcam | 133616 | 3000 | 0.05 | Tris-EDTA | TSA555 | o/n | IC |
CD8 | ms | BioSB | 5174 | 100 (1000) | N/A LOT5174JDL05 | Tris-EDTA | AlexaFluor750 | 90 min | IC |
CD45 | rbt | CellSignalling | 13917 | 100 (500) | N/A, LOT 1 | Tris-EDTA | AlexaFluor647 | 90 min | IC |
Ki67 | rbt | Abcam | 92742 | 5000 | 0.14 | Tris-EDTA | Liquid Permanent Red (AP) | 90 min | IC |
E-cadherin | ms | BD biosciences | 610182 | 10000 | 0.03 | Tris-EDTA | VinaGreen (HRP) | 90 min | IC |
PanCK | ms | Abcam | 7753 | 2000 | 0.5 | Tris-EDTA | VinaGreen (HRP) | 90 min | IC |
PanCK | ms | Invitrogen | 18–0132 | 2000 | 0.02 | Tris-EDTA | VinaGreen (HRP) | 90 min | IC |
1Brackets indicate the optimized dilution for chromogenic/TSA-amplified detection.