Table 1.

Primary antibodies, fluorochromes, and chromogens used in the study. All antibodies are commercially available. See also Supplementary Figure S5 for primary antibody denaturation testing. AMACR, alpha-methylacyl-CoA racemase; AR, androgen receptor; CK, cytokeratin; EPI, epithelial; HIER, heat-induced epitope retrieval; HRP, horse-radish peroxidase; IC, immune cell; ms, mouse; o/n, overnight; rbt, rabbit; TSA, tyramide signal amplification.

Target	Species	Vendor	Cat number	Dilution1	Concentration (µg/ml)	HIER buffer	Label (enzyme)	Incubation	Panel
CK18	ms	SantaCruz	6259	2000	0.1	Tris-EDTA	TSA488	o/n	EPI
CK8	ms	Invitrogen	18–0185z	2000	0.11	Tris-EDTA	TSA555	o/n	EPI
p63	rbt	Abcam	124762	1000	0.87	Tris-EDTA	AlexaFluor647	90 min	EPI
CK5	rbt	Abcam	52635	400 (2000)	0.88	Tris-EDTA	AlexaFluor647	90 min	EPI
E-cadherin	ms	BD biosciences	610182	1000 (10000)	0.25	Tris-EDTA	AlexaFluor750	90 min	EPI
PanCK	ms	Abcam	7753	200 (2000)	5	Tris-EDTA	AlexaFluor750	90 min	EPI
PanCK	ms	Invitrogen	18–0132	200 (2000)	0.18	Tris-EDTA	AlexaFluor750	90 min	EPI
AR	rbt	SantaCruz	13062	2000	0.1	Tris-EDTA	VinaGreen (HRP)	90 min	EPI
AMACR	ms	Abcam	63340	2000	N/A, LOT GR154665-12	Tris-EDTA	Liquid Permanent Red (AP)	90 min	EPI
FoxP3	ms	Abcam	20034	500	2	Tris-EDTA	TSA488	o/n	IC
CD4	ms	Abcam	133616	3000	0.05	Tris-EDTA	TSA555	o/n	IC
CD8	ms	BioSB	5174	100 (1000)	N/A LOT5174JDL05	Tris-EDTA	AlexaFluor750	90 min	IC
CD45	rbt	CellSignalling	13917	100 (500)	N/A, LOT 1	Tris-EDTA	AlexaFluor647	90 min	IC
Ki67	rbt	Abcam	92742	5000	0.14	Tris-EDTA	Liquid Permanent Red (AP)	90 min	IC
E-cadherin	ms	BD biosciences	610182	10000	0.03	Tris-EDTA	VinaGreen (HRP)	90 min	IC
PanCK	ms	Abcam	7753	2000	0.5	Tris-EDTA	VinaGreen (HRP)	90 min	IC
PanCK	ms	Invitrogen	18–0132	2000	0.02	Tris-EDTA	VinaGreen (HRP)	90 min	IC

¹Brackets indicate the optimized dilution for chromogenic/TSA-amplified detection.