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. 2017 Autumn;8(4):311–316. doi: 10.22088/cjim.8.4.311

Table 1.

Specific primers for detection of mecA and pvl genes and SCCmec various types

Reference Product size(bp) Sequence(5-3) Primer Target
(7) 533 AAAATCGATGGTAAAGGTTGGC
AGTTCTGCAGTACCGGATTTGC
F
R
mecA
(8) 433 ATCATTAGGTAAAATGTCTGGACATGATCCA GCATCAACTGTATTGGATAGCAAAAGC F
R
Pvl
(9) 937 ATTGCCTTGATAATAGCCYTCT
TAAAGGCATCAATGCACAAACACT
Fb
Ra3
Sccmec
518 CGTCTATTACAAGATGTTAAGGATAAT
CCTTTATAGACTGGATTATTCAAAATAT
FccrC
RccrC
415 GCCACTCATAACATATGGAA
CATCCGAGTGAAACCCAAA
F1272
R1272
359 TATACCAAACCCGACAACTAC
CGGCTACAGTGATAACATCC
F5RmecA R5R431

mecA: methicillin-resistant gene, pvl: Panton-Valentine leukocidin, Sccmec: Staphylococcal cassette chromosome mec