Table 2.

PCR programs for detection of mecA and pvl genes and SCCmec various types

Final extention	Extention	Annealing	Denaturation	Initial enzyme activation	Cycle	Gene
72 for 5 min	72 for 90 sec	50 for 30 sec	94 for 1 min	94 for 5 min	30	mecA
72 for 5 min	72 for 1 min	55 for 30 sec	94 for 30 sec	95 for 5 min	30	pvl
72 for 4 min	72 for 1 min	55 for 30 sec	94 for 30 sec	94 for 5 min	30	SCCmec

mecA: methicillin-resistant gene, pvl: Panton-Valentine leukocidin, Sccmec: Staphylococcal cassette chromosome mec