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. 2017 Aug 11;24(12):2032–2043. doi: 10.1038/cdd.2017.125

Figure 5.

Figure 5

Reduction in MCL-1 levels does not drastically exacerbate haematopoietic cytopenia caused by non-DNA-damaging chemotherapeutic drugs. Wild-type (wt) and Mcl-1+/− mice were treated with dexamethasone (3 × 10 mg/kg body weight, i.v.; wild-type n=8; Mcl-1+/− n=13) or paclitaxel (3 × 3 mg/kg body weight, i.v.; wild-type n=9; Mcl-1+/− n=11) and monitored for a total of 35 days (dexamethasone) or 21 days (paclitaxel). A subset of animals of each cohort was killed 7 days post-treatment for detailed flow cytometric and histological analysis. (a) Lymphocyte and (b) RBC numbers were determined in peripheral blood at the indicated time points post-treatment with dexamethasone (left panel) or paclitaxel (right panel). Data represent mean ±S.E.M. *P<0.05, **P<0.01, ***P<0.001 (Student's t-test, two tailed, paired, compared with untreated). (c) Histological analysis of H&E-stained sections of the bone marrow (sternum) of wild-type and Mcl-1+/− mice 7 days post-treatment with dexamethasone (upper panel) or paclitaxel (lower panel). Flow cytometric analysis of the indicated bone marrow (BM) cell populations (d) and the indicated B-cell populations (e) (total cell count for one femur) in wild-type and Mcl-1+/− mice 7 days post-treatment with dexamethasone (upper panel; wild-type n=3; Mcl-1+/− n=3) or paclitaxel (lower panel); wild-type n=3; Mcl-1+/− n=3) compared with untreated wild-type (n=7) and Mcl-1+/− mice (n=7). Data represent mean ±S.E.M. P>0.5 (n.s.) (Student's t-test, two tailed, unpaired, comparing wild-type with Mcl-1+/− mice). (f) Flow cytometric analysis of total thymic T cells in wild-type and Mcl-1+/− mice 7 days post-treatment with dexamethasone (left panel; wild-type n=3; Mcl-1+/− n=3) or paclitaxel (right panel; wild-type n=3; Mcl-1+/− n=3) compared with untreated wild-type (n=7) and Mcl-1+/− mice (n=7). DN=double negative; DP=double positive; SP=single positive thymocytes. Data represent mean ±S.E.M. P>0.5 (n.s.) (Student's t-test, two tailed, unpaired, comparing wild-type with Mcl-1+/− mice)