Figure 7.

Treatment of Nedd4-2^Ksp1.3 mice with diuretic drugs to block ENaC and NCC variably affects kidney damage. (a) Time course of treatment from P20 until P40. Drugs were added to the drinking water daily: amiloride (2 mg/kg) and HCTZ (10 mg/kg). (b) Blood aldosterone levels are shown for all treatment groups at P40. Amiloride treatment increases levels of aldosterone, as expected. (c) qRT-PCR data showing Vimentin and Kim-1 expression levels in control and Nedd4-2^Ksp1.3 kidneys, administered with diuretic drugs in the tap water. (e and f) Representative images of Kim-1 immunostaining showing areas of kidney damage in the cortex in untreated and treated mice (e), and the result of quantitation (f). (b–d) n=9–14. (f) Samples were randomly selected from drug treatment experiment; n=4–6 for control untreated and Nedd4-2^Ksp1.3 amiloride and HCTZ treated, n=11 for Nedd4-2^Ksp1.3 untreated (one dot represents one mouse). *P<0.05, **P<0.01, ***P<0.005 and ****P<0.001. Data are presented as mean±s.d. One-way analysis of variance (ANOVA) with Bonferroni’s post-test for comparison between multiple groups was used. Scale bar: (c)=500 μm