Figure 5.

Restoring miR-148a expression attenuates spontaneous and AOM/dextran sodium sulfate (DSS)-induced tumorigenesis. (a) Body weight changes of WT and miR-148a KO mice treated with lentivirus containing Pri-miR-148a or control expression vector during AOM/DSS treatment (n=9–12). (b) Typical images of colon tumors of mice from (a) 120 days after AOM/DSS treatment. (c and d) Colon tumor number (c, Left), averge volume (c, right) and size (d) in mice from panel (b). (e) Inhibition of NF-κB and STAT3 signaling was determined in the colons collected in mice from panel (b). Each lane represents an individual mouse. (f) Cytokine levels were determined by ELISA in colon tissues collected in mice from panel (b). (g and h) Colon tumor number (g, Top), average volume (g, Bottom) and size (h) in Apc^min/+ mice treated with lentivirus containing Pri-miR-148a or a control expression vector. (i) Small intestine tumor number (Top) and average volume (Bottom) in Apc^min/+ mice from panel (g) (n=9–10). Data present mean±S.D. in panels (a, d and h). *P<0.05, **P<0.01, ***P<0.001