Figure 2.
4-Hydroxytamoxifen (TAM) induction of Tg endoR2−/− partially restores lymphocyte development. Young adult Tg-endoR2−/− mice (Tg endoR2−/−) were fed with normal (−) or TAM (+) food during 4 weeks before analysis. Untreated wild-type (WT) and recombination activating gene 2 (RAG2)-deficient (R2−/−) mice served as control. (A) Representative FACS profiles of thymocytes, bone marrow, and lymph nodes cells. (B) Full analysis of thymocytes numbers and frequency of thymic DP (upper panels); frequency of E and F fractions and CD43+ B cells in the bone marrow (middle panels); concentration of seric Ig and lymphocyte numbers in lymph nodes (bottom panels). *, **, and *** stand for p-values <10−2, 10−3, and 10−5, respectively. Results are representative of six independent experiments.