Table 1. Primer sequences.
Type | Primer name | Sequences | |
---|---|---|---|
Human | MDR1 | Forward | 5′-CCC ATC ATT GCA ATA GCA GG-3′ |
Reverse | 5′-GTT CAA ACT TCT GCT CCT GA-3′ | ||
Human | MRP1 | Forward | 5′-GCC GAA GGA GAG ATC ATC-3′ |
Reverse | 5′-AAC CCG AAA ACA AAA CAG G-3′ | ||
Human | MRP2 | Forward | 5′-CGA CCC TTT CAA CAA CTA CTC-3′ |
Reverse | 5′-CAC CAG CCT CTG TCA CTT C-3′ | ||
Human | MRP3 | Forward | 5′-GTG GGG ATC AGA CAG AGA T-3′ |
Reverse | 5′-TAT CGG CAT CAC TGT AAA CA-3′ | ||
Human | MRP4 | Forward | 5′-GCT CAG GTT GCC TAT GTG CT-3′ |
Reverse | 5′-CGG TTA CAT TTC CTC CTC CA-3′ | ||
Human | MRP5 | Forward | 5′-CAG CCA GTC CTC ACA TCA-3′ |
Reverse | 5′-GAA GCC CTC TTG TCT TTT TT-3′ | ||
Human | MRP6 | Forward | 5′-AGG AGG CCC GAG CTT AGA C-3′ |
Reverse | 5′-CCT GCC CTA TTG GAT GCT GT-3′ | ||
Human | MRP9 | Forward | 5′-ATG CGG TTG TCA CTG AAG-3′ |
Reverse | 5′-GTT GCC TCA TCC ATA ATA AGA AT-3′ | ||
Human | GAPDH | Forward | 5′-CGT CTT CAC CAC CAT GGA GA-3′ |
Reverse | 5′-CGG CCA TCA CGC CAC AGT TT-3′ |
RT-PCR was performed by coamplification of the genes with a reference gene by use of the cDNA template and corresponding gene-specific primer sets