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. 2017 Sep 22;14(6):6571–6577. doi: 10.3892/ol.2017.7023

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Copyright: © Lee et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Effect of TCS7010 on the upregulation of CHOP and BIM proteins. (A) HCT116 cells were treated with 5 µM TCS7010 for various time periods (0–24 h). Whole-cell lysates were subjected to immunoblotting using antibodies directed against CHOP and BIM. GAPDH was used as an internal control. Immunoblot band intensities were analyzed using a quantitative scanning densitometer (right graph). Vehicle (DMSO)-treated band intensity was set to 1 and the relative values of fold-increase were plotted as the mean ± standard deviation (n=3). Statistical analysis was performed using analysis of variance followed by Sidak's multiple comparisons test. HCT116 cells were treated with 5 µM TCS7010 for (B) 6 h or (C) 12 h in the absence or presence of 2 mM NAC. Whole-cell lysates were subjected to immunoblotting. GAPDH was used as an internal control. Immunoblot band intensities were analyzed using a quantitative scanning densitometer (right graph). Vehicle (DMSO)-treated band intensity was set to 1 and the relative values of fold-increase were plotted as the mean ± SD (n=3). Statistical analysis was performed using analysis of variance followed by Sidak's multiple comparisons test. Cl., cleaved; Casp, caspase; NAC, N-acetylcystein.