Table III.
Effect of P1 on apoptosis induced by DOX.
| A, MOLT4 cells | ||||
|---|---|---|---|---|
| P1, nmol/ml | ||||
| Treatment | 0 | 0.1 | 1.0 | |
| Control | 5.3±0.6 | 4.8±0.5 | 6.1±1.0 | |
| DOX | 52.4±2.5 | 64.7±3.1a | 79.5±4.2b | |
| B, RBL-2H3 cells | ||||
| P1, nmol/ml | ||||
| Treatment | 0 | 0.1 | 1.0 | |
| Control | 4.3±0.5 | 6.6±0.8 | 5.9±0.4 | |
| DOX | 50.1±3.0 | 52.3±2.6 | 51.5±2.9 | |
The effect of P1 on cell apoptosis induced by DOX was determined using fluorescence-activated cell sorting. Values are percentages. (A) For MOLT4 cells, P1 significantly increased number of apoptotic cells induced by DOX
a
P<0.05 (0.1 nmol/ml P1 and DOX group vs. only DOX group)
b
P<0.01 (1.0 nmol/ml P1 and DOX group vs. only DOX group). (B) For RBL-2H3 cells, no significant difference between groups treated with P1 and DOX compared with only DOX was identified. The data are presented as the mean ± standard deviation. The experiments were carried out three times. DOX, doxorubicin.