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. 2017 Oct 1;23(10):575–582. doi: 10.1089/ten.tec.2017.0244

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© Adam G.E. Day et al. 2017; Published by Mary Ann Liebert, Inc.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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FIG. 3. — Metabolic activity of EngNT with and without preservation in Hibernate-A. After 3 days in Hibernate-A, preserved samples were put back into culture conditions for 1 day (4 days total time) or 3 days (6 days total) and compared with equivalent samples maintained in culture for 4 and 6 days. Two-way ANOVA revealed a main effect between conditions (p < 0.05), time (p < 0.001), and their interaction (p < 0.05). Two-way ANOVA with Tukey's post-tests showed that control samples (analyzed immediately after construction) had significantly higher metabolic activity than all other samples (dotted line, 186,000 ± 8000; p < 0.001). The hypothermic condition at day 4 had significantly higher metabolic activity than the other samples at days 4 and 6 (**p < 0.01, ***p < 0.001). n = 4, data are mean ± SEM.