Figure 4.

ADAM9 is a direct target gene of miR-154. (A) Schematic of a putative miR-154 binding site in the 3′UTR of ADAM9. (B) Luciferase reporter assays exhibited a reduction in the relative luciferase activities of PmirGLO-ADAM9-3′UTR Wt following transfection with an miR-154 mimic. (C) Reverse transcription-quantitative polymerase chain reaction analysis of ADAM9 mRNA expression levels in MCF-7 and MDA-MB-231 cells transfected with an miR-154 mimic or NC. (D) Western blot analysis of ADAM9 protein expression levels in MCF-7 and MDA-MB-231 cells transfected with an miR-154 mimic or NC. *P<0.05 compared with NC. ADAM9, ADAM metallopeptidase domain 9; miR, microRNA; 3′UTR, 3’-untranslated region; Wt, wild-type; Mut, mutant.