. 2017 Nov 14;8(6):e00824-17. doi: 10.1128/mBio.00824-17

TABLE 1 .

Results obtained for the species-specific detection of Methanoarchaea in gut biopsy samples of healthy controls and UC and CD patients^a

Target or parameter	Healthy controls					Inflamed UC					Uninflamed UC					Inflamed CD					Uninflamed CD
Target or parameter	1^b	2	3	4	5	1	2	3	4	5	1	2	3	4	5	1	2	3	4	5	1	2	3	4	5
M. smithii	−/+/+	−	+/+/+	−	−	−/+/−	+/+/+	+/+/−	+/−/−	+/+/−	−/+/−	+/−/−	−/+/−	−/−/−	−/+/−	+/+/−	+/−/+	+/+/−	+/−/−	−/+/+	+/−/−	+/+/−	−/+/−	−/+/−	−/+/−
M. stadtmanae	−/+/−	−/−	−/+/−	−/−	−/+	−/−/−	−/−/+	−/−/−	−/−/−	−/−/−	+/−/−	−/+/+	−/−/−	−/−/−	−/−/−	−/−/−	+/−/−	−/−/−	−/−/−	−/+/−	−/−/−	−/+/−	−/−/−	−/−/−	−/−/−
M. luminyensis	−/−/−	−/−/−	+/−/−	−/−/−	+/−/−	−/−/−	−/−/−	−/−/−	−/+/−	−/−/−	−/−/−	−/−/−	−/−/−	−/−/−	−/−/−	−/−/−	+/+/−	+/−/−	+/−/−	−/−/−	−/−/−	−/−/−	−/−/−	−/−/−	−/−/−
Archaea^c	−/−/−	−/+/−	+/+/+	−/−/−	+/−/+	+/+/+	−/+/−	−/−/−	−/−/+	−/−/−	−/−/−	−/+/+	−/+/+	−/−/−	−/+/+	−/+/−	−/+/−	−/+/−	−/−/−	−/−/+	−/−/+	+/+/+	+/+/−	−/+/−	−/−/−
Identity^d		M. smithii	M. smithii, M. stadtmanae		Euryarchaeote SCGC AAA003-G23	M. smithii	M. smithii, M. stadtmanae		M. stadtmanae			M. smithii	M. smithii, M. stadtmanae		M. smithii	M. smithii	M. smithii, M. stadtmanae	M. smithii		M. stadtmanae	M. smithii, M. stadtmanae	M. smithii	M. stadtmanae	M. smithii

^{^a}

Nested PCR of the rpoB gene (and of the 16S rRNA gene as a control) was performed as described in Materials and Methods. Positive (+) and negative (−) results out of three replicates are shown (each symbol reflects one replicate). Where only one or two symbols are shown, three replicates could not be analyzed because of a low DNA yield.

^{^b}

Biopsy sample number.

^{^c}

16S rRNA gene.

^{^d}

Obtained by Sanger sequencing.