Figure 5. Binding of Atg18^Sloop and Atg18^Dloop to small unilamellar vesicles.

• A–D
  Binding of recombinant Atg18 variants to liposomes. SUVs were made of EPC with 5% of the indicated phosphoinositides, or of EPC and the indicated fraction of PS. Cholesterol had been added to 20 mol% of the total phospholipid amount. Liposomes were incubated with the purified proteins for 30 min at room temperature (25°C) and centrifuged, and the supernatants (S) and pellets (P) analyzed by SDS–PAGE and Coomassie staining. Binding is shown for (A) Atg18^wt; (B) Atg18^FGGG; (C) Atg18^DLoop; (D) Atg18^SLoop.
• E
  The bands from (A–D) were quantified from three independent experiments. The mean with SEM is shown.