Figure EV1. Fractionation of the bleb‐membrane proteins.

1. SDS–PAGE electrophoresis of the protein fractions obtained after preparative liquid‐phase isoelectric focusing.
2. Maxi‐Cl activity in each fraction after reconstitution into the giant liposomes. Number of patches tested is shown at the top of each column. No proteoliposomes could be formed using fraction‐1.
3. The gel for F7 was divided into 26 pieces for the LC‐MS/MS analysis.

Source data are available online for this figure.