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. 2017 Oct 18;36(22):3309–3324. doi: 10.15252/embj.201796685

Figure EV3. Gd3+ and BSP sensitivity of non‐Maxi‐Cl channels in SLCO2A1‐deficient cells.

Figure EV3

  1. Mean patch currents recorded at +25 mV in the outside‐out mode in the absence (Control: white column) and presence of Gd3+ (50 μM: red column) in the bath solution. Error bars, SEM, *P < 0.05 (Student's t‐test). n = 5 (from different cells) for Control and Gd3+.
  2. Dose‐dependent inhibitory effects of BSP (added at bars) on currents recorded from an inside‐out patch in response to a step‐pulse of ±25 mV applied every 5 s. Result of the representative experiment is shown.
  3. Concentration–response curves of BSP effects with IC50 = 8.3 ± 1.7 μM at +25 mV and 5.2 ± 0.4 μM at −25 mV. The inset on top shows flickery currents observed in the presence of 3 μM BSP. n = 5 for each data point (from five different experiments as shown on panel B). Error bars, SEM.