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. 2017 Sep 28;36(22):3292–3308. doi: 10.15252/embj.201696056

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© 2017 The Authors. Published under the terms of the CC BY 4.0 license

This is an open access article under the terms of the Creative Commons Attribution 4.0 License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Figure EV1 — Genomic PCR analysis of Cre recombination in MACS‐sorted splenic dendritic cells (DC) from PN21 Igf1^wt/fl; DC, astrocytes from PN21 CD11c^Cre‐GFP Igf1^fl/fl (homozygous) as well as microglia, neurons, OPC, and astrocytes from CD11c^Cre‐GFP Igf1^wt/fl (heterozygous). Wild‐type Igf1 gene is detected as an ~1‐kb band; Cre‐induced recombination is detected as an ~0.2‐kb band, while the Igf1/flox locus cannot be amplified under the assay condition (Liu et al, 1998).