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. 2017 Sep 28;36(22):3292–3308. doi: 10.15252/embj.201696056

Figure 5. Neuroectodermal marker expression in neonatal microglia.

Figure 5

  • A
    A heatmap showing neuroectodermal gene expression in neonatal versus adult microglia. Scale represents log2 fold change normalized CPM expression values.
  • B, C
    Expression of nestin in MACS‐sorted microglia (n = 4), OPC (n = 4), astrocytes (n = 4), and neurons (n = 3) from brains of PN3‐7 mice (B) as well as FACS‐sorted CD11c+ microglia (n = 5), CD11c− microglia (n = 5) from brains of PN4‐7 mice, and total microglia from adult brain (C).
  • D, E
    Representative flow cytometry profiles (D) and bar graph (E) showing nestin expression in microglia from brains of PN5 mice (n = 5) and adult mice (n = 6).
  • F, G
    Confocal microscopic analysis showing co‐localization of nestin (red) (F) or GFAP (red) (G) with IBA1 (green) (n = 3). Arrows point to IBA1, NES (F) and IBA1, GFAP (G) double positive cells. Scale bars = 15 μm.
Data information: Data are based on at least two experimental repeats. Data are presented as means ± SEM; each n represents an individual mouse. P‐values were determined by two‐tailed Mann‐Whitney U‐test. ns, not significant; *P < 0.05; **P < 0.01.