FIG 4.
mgtE expression affects Rsm signaling by a GacAS-dependent mechanism. Transcriptional rsmY (PrsmY-lacZ) (A), rsmZ (PrsmZ-lacZ) (B), and rsmA (PrsmA-lacZ) (C) reporter strains in the WT, ΔgacA, and ΔgacS backgrounds with either the vector control or pmgtE were assayed under T3SS-inducing (+EGTA) conditions for β-galactosidase activity. The percentage of activity was calculated considering the lacZ activity from the respective WT reporter strains with the blank vector as 100%. *, P < 0.01; n.s., not significant (ANOVA).