FIG 4.

CueP can contribute to activation of SodCI and SodCII. SOD assays were performed using the xanthine oxidase-cytochrome c method. Cultures were grown overnight in 10 ml LB with TETA when indicated. Strains were devoid of all native SOD and expressed only the listed SodC from a pWSK29 vector. The osmotic shockates (A and B) or whole-cell extracts (C) were remetalated with 45 μM CuSO₄. Data shown are the initial SOD activity divided by the SOD activity after remetalation and are representative of three independent experiments. The strains used were JS2093 and JS2094 containing either pMR101 or pMR102.