FIG 4.

DivIVA binding of MinD is MinJ independent. Western blots showing pulldown eluates obtained with MinD-His as the bait protein in wild-type (LMJR86) and ΔminJ mutant backgrounds (LMJR85). Strains expressing MinJ-His (LMJR84, positive control) and SecA2-His (LMKK3, negative control) were included. DivIVA was detected using a polyclonal antiserum raised against B. subtilis DivIVA (left blot), and His-tagged proteins were detected using an anti-His antibody (right blot). EGD-e (wt) was included as another negative control. Please note that MinJ-His (45.5 kDa) runs at an aberrant position after cross-linking and heat treatment (26).