TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| Plasmids | ||
| pIMK3 | Phelp-lacO lacI neo | 36 |
| pMAD | bla erm bgaB | 38 |
| pJR55 | Phelp-minD-his10 neo | 26 |
| pSH2 | bla aprE5′ spc lacI Pspac-divIVABs aprE3′ | 16 |
| pSH19 | bla amyE3′ spc Pxyl-divIVABs amyE5′ | 18 |
| pSH186 | bla erm bgaB ΔdivIVA (lmo2020) | 25 |
| pSH260 | bla amyE3′ spc Pxyl- divIVALm-71-Bs amyE5′ | 18 |
| pSH221 | Phelp-lacO-divIVALm lacI neo | 25 |
| pKK16 | Phelp-lacO-divIVABs lacI neo | This work |
| pKK17 | Phelp-lacO-divIVALm-104-Bs lacI neo | This work |
| pKK18 | Phelp-lacO-divIVALm-57-Bs lacI neo | This work |
| pKK21 | Phelp-lacO-divIVABs-57-Lm lacI neo | This work |
| pKK90 | bla erm bgaB divIVALm | This work |
| pSH388 | Phelp-lacO-divIVABs-17-Lm-104-Bs lacI neo | This work |
| pSH394 | Phelp-lacO-divIVALm-71-Bs lacI neo | This work |
| pSH472 | bla erm bgaB divIVAD30A | This work |
| pSH473 | bla erm bgaB divIVAY20F | This work |
| pSH474 | bla erm bgaB divIVAI33A | This work |
| L. monocytogenes strains | ||
| EGD-e | Wild-type serovar 1/2a strain | Lab collection |
| LMJR84 | attB::Phelp-minJ-his10 neo | 26 |
| LMJR86 | attB::Phelp-minD-his10 neo | 26 |
| LMKK3 | attB::Phelp-secA2-his10 neo | 26 |
| LMKK30 | attB::Phelp-gfpA206K-minD neo | 26 |
| LMKK35 | ΔminCD | 26 |
| LMKK54 | ΔminCDJ | 26 |
| LMS2 | ΔdivIVA | 25 |
| LMS30 | ΔdivIVA attB::Phelp-lacO-divIVALm lacI neo | 25 |
| LMS120 | ΔminJ | 26 |
| LMS148 | ΔminC | 26 |
| LMJR85 | ΔminJ attB::Phelp-minD-his10 neo | This work |
| LMKK8 | ΔdivIVA attB::Phelp-lacO-divIVABs lacI neo | This work |
| LMKK12 | ΔdivIVA attB::Phelp-lacO-divIVALm-57-Bs lacI neo | This work |
| LMKK13 | ΔdivIVA attB::Phelp-lacO-divIVALm-104-Bs lacI neo | This work |
| LMKK14 | ΔdivIVA attB::Phelp-lacO-divIVABs-57-Lm lacI neo | This work |
| LMS149 | ΔdivIVA attB::Phelp-lacO-divIVABs-17-Lm-104-Bs lacI neo | This work |
| LMS157 | ΔdivIVA attB::Phelp-lacO-divIVALm-71-Bs lacI neo | This work |
| LMS196 | divIVAY20F | This work |
| LMS197 | divIVAI33A | This work |
| LMS198 | divIVAD30A | This work |
| LMS227 | divIVAI33A attB::Phelp-gfpA206K-minD neo | This work |